Published Papers
Strategies for the Codelivery of Osteoclasts and Mesenchymal Stem Cells in 3D-Printable Osteochondral Scaffolds
Jabari, E., Choe, R., Kuzemchak, B., Venable-Croft, A., Choi, J.Y., McLoughlin, S., Packer, J.D., Fisher, J.P.Tissue Engineering Part C: Methods. (2024).
We examined the growth of a co-culture of mesenchymal stem cells (stem cells for bone and cartilage growth) and osteoclasts (cells that resorb bone). This experiment produced an enormous amount of images of cell cultures.
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My Contribution: I developed computer vision software for the automated extraction of features to make data analysis easier on my fellow researchers. I extracted cell counts, calcium and collagen protein deposition (bone formation markers), and osteoclast development in bright-field and fluorescent images.
​3D printable phantom for mimicking electrical properties of dermal tissue
Kuzemchak BC, Choe RH, Sherry M, Porter E, Fisher JP. Journal of Biomedical Materials Research: Part A. (2023).
This is my lead-author manuscript that won a national award at the Society for Biomaterials. I designed and executed all experiments. I created a 3D-printable material that mimics the electrical conductivity and permittivity of skin. This material can be used to create complex 3D models of skin cancer tumors to develop electrical-based devices for skin cancer detection. This experiment created a lot of data points (2592 to be exact) so I created software to automatically process the data and compress it to 270 points using a mathematical model.
Designing Biomimetic 3D-Printed Osteochondral Scaffolds for Enhanced Load-Bearing Capacity
Choe RH, Kuzemchak BC, Kotsanos GJ, Mirdamadi E, Sherry M, Devoy E, Lowe T, Packer JD, Fisher JP. Tissue Engineering Part A. (2024)
Contribution: We use 3D-printed fibrous scaffolds to encourage the growth of mesenchymal stem cells in cartilage. These scaffolds are meant to be implanted into the knee to regenerate lost cartilage and therefore have to be examined for mechanical failure due to the high physiological forces present in this joint.
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My Contribution: I created a mathematical model of compressive mechanical failure in the implant fiber arrays. I incorporated buckling and yielding failure modes and examined the effect 3D printing has on the implant's compressive strength. I also created software to automate the processing of non-linear stress-strain curves. This was one of my favorite research projects.
Computational investigation of interface printing patterns within 3D printed multilayered scaffolds for osteochondral tissue engineering
Choe RH, Devoy E, Kuzemchak B, Sherry M, Jabari E, Packer JD, Fisher JP. Biofabrication. (2022).
We developed an implant to regenerate the interface between cartilage and bone. The osteochondral interface is a critical area for joint operation. It is a complex interconnection of multiple tissues with very different mechanical stiffnesses. We multilateral 3D printed a hard polymer (to mimic bone) and different hydrogel formulations (to mimic cartilage) in a hybrid implant and assessed the shear failure between polymer and hydrogel layers.
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My Contribution: I developed the material formulations for the bioinks, manufactured the hybrid implants, and performed shear force failure analysis.
Presentations
“Under pressure”: Understanding How Hydrostatic Pressure (HP) Influences the Stromal-Hematopoietic Stem Cell Interplay
Erfan Jabari, Blake Kuzemchak, Anastasia du Halgouet, Isabella Conway, Grozdan Cvijetic, Joanne Shi, Valentina Ottaviani, Harrison Wang, Anjali Chandroth, Siqi Zhao, *John P. Fisher and Roxane Tussiwand
Biomedical Engineering Society (2024)
